Molecular variability in isolates of Rhizoctonia bataticola causing root rot in chickpea by SSR marker

Abstract

The Genetic diversity of all the isolates of Rhizoctonia bataticola was analysed by using Simple Sequence Repeat (SSR) markers. The SSR primers were tested for amplification of genomic DNA of Rhizoctonia bataticola isolates. The SSR analysis total 8 primers were screened against eight isolates of Rhizoctonia bataticola. Out of 8 primers 4 primers amplified scorable banding pattern. Out of 59 bands, 46 bands were polymorphic and average level of Polymorphism was 77.96% in the dendrogram, Rb-1 (Akola) was found to have higher value of similarity coefficient (0.723) whereas Rb-3 (Amravati) was found to have lower value of similarity coefficient (0.447). The isolates of Rhizoctonia bataticola were grouped into four major clusters. First group is named as cluster-A, includes isolates belonging to Akola, Amravati. Second group is named as cluster-B, includes Nagpur and Kurundwad, third group is named as cluster-C, includes Umbraj, Karanje and Sangli and fourth group is named as cluster D-includes Kini. The similarity matrix indicated that eight isolates of Rhizoctonia bataticola exhibited (44.7- 72.30) per cent similarity coefficient.