Pharmacokinetics of cefquinome and cefquinome concentration in various biological fluids in Marathwadi buffaloes

Abstract

Experiment was performed on six healthy Marathwadi buffaloes to study the different pharmacokinetic parameters and cefquinome concentration in various biological fluids after single intramuscular @ 1 mg/kg body weight by microbiological assay technique. The peak serum concentration, absorption half-life, distribution half life, elimination half life, volume of distribution, total body clearance, AUC, AUMC, MRT and bioavailability values of cefquinome after intramuscular administration found were 25.67 ± 4.65 mcg/ml, 0.21 ± 0.02h, 0.39 ± 0.05 h, 2.30 ± 0.12 h, 0.13 ± 0.02 L/kg (Vd(B)) and 0.14±0.02 L/kg (Vdss), 0.04 ± 0.00 L/kg.h-1,27.24±3.67µg/ml.hr, 92.43 ± 13.30µg /ml.hr2, 3.40± 0.19 h and 177.22%, respectively. The cefquinome concentration in saliva, nasal secretion, urine and milk was recorded as 0.29 ± 0.01, 0.33 ± 0.01, 2.60 ± 0.35 and 0.258 ± 0.01 mcg/ml respectively at twelve hours, however at the same time cefquinome concentration in serum could not be detected. It may be concluded that Cefquinome persists for 10 hours in buffaloes with the peak concentrations 0.76 hours after IM administration. The elimination half life of cefquinome was 2.3 h in adult buffaloes indicating the repeating of doses at 12 to 15 h intervals in buffaloes. The loading dose would be double than the maintenance dose of cefquinome after intramuscular administration.