Effect of sterilants on shoot regeneration of Banana (Musa acuminata) cv. Monthan in vitro plantlets

Abstract

In vitro culture of plant species most commonly depends on the optimal size of explants used for the purpose of study. Relatively, larger explants (3-10 mm) are desirable for rapid multiplication despite of its higher susceptibility to blackening and contamination. The surface sterilization of banana shoot-tips explants of desired size was performed before transferring them to the culture media to make our cultures contamination free. In the present study, different sizes of explants and different sterilants were used for determining the growth parameters of cv. Monthan and to check their ability of making a media contamination free. The toxicity percentage was seen to be dependent on the sterilants concentration and duration of time for being they are used. Double disinfection method was also used in case of longer persistence of contamination.