Molecular characterization of soybean genotypes in response to charcoal rot disease by using RGA markers

Abstract

Charcoal rot disease caused by Macrophomina phaseolina is one of the most damaging diseases of soybean resulting to 70 % losses and till date no immune genotype is known for the same.Charcoal rot (CR) disease caused by Macrophomina phaseolina is responsible for significant yield losses in soybean production. The present study helped to evaluate soybean genotypes for identifying promising genotypes which proved to be resistant to charcoal rot. The present study was carried out at Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during the year 2018-19 to evaluate various genotypes of soybean for charcoal rot resistance. Among the methods available for controlling this disease, breeding for resistance is the most promising. Molecular characterization of these genotypes was done by using RGA markers. Molecular profiles revealed remarkable polymorphism and observations showed that in total 38 amplicons were tested with an average of 5.42 alleles per locus. Out of the total screened alleles 13 were monomorphic alleles with an average of 1.85 and 25 were polymorphic alleles with an average of 3.57. Results showed an average of 65.03 polymorphism percent. The PIC (Polymorphic information content) value ranged from 0.43 to 0.83 with an average value of 0.62, these studies will help in mapping studies and breeding program for development of charcoal rot resistance in soybean genotypes which will be of utmost importance.