In vitro regeneration of Bacopa monnieri (L.) from leaf and stem explants

Abstract

The effects of plant growth regulators (PGRs) and activated charcoal (AC) on plant regeneration and plantlets development were assessed in vitro using the leaf and stem explants of Bacopa monnieri Leaf and stem explants were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of PGRs (Auxins and cytokinin) with and without Activated charcoal AC) (0.5 to 2.5 g/L). Plant regenerated successfully through calli derived from stem and leaf plants. Compared to stem, leaf explants showed good callus formation. Maximum callus formation (92.23%) was recorded for naphthalene acetic acid (NAA) (3 mg/L) whereas, 73.11% callus formation was recorded for 2,4-D (2.5 mg/L). A combination of IBA+BAP (3 mg/L) showed highest shoot formation and multiple shoot formation by 76.43% and 49.55%, respectively whereas, addition of IBA (2 mg/L) showed 73.23% and 47.24% shoot formation and multiple shoot formation, respectively. Addition of AC with IBA (2 mg/L) exhibited maximum root formation by 85.52% and number of roots per shoot by 38.55% whereas, with IAA (2 mg/L), the root formation was found to be 71.47% and number of roots per shoot to be 33.44% The present study concluded that addition of AC promotes roots induction and formation from in vitro shoots in Brahmi.