In vivo histopathological and in vitro cell culture studies upon Canine parvovirus infection

Abstract

In the present study in vivo and in vitro studies were carried out on Canine parvovirus (CPV). Crandell rees feline kidney and Madin darby canine kidney cell lines were used for the propagation of the virus in the laboratory and tissues from the parvo affected dogs were collected after the post mortem and histopathological changes were noticed. A total of 9 carcasses infected with parvovirus were used for the in vivo studies and important infected organs like intestines and heart histopathological sections were done to identify the changes produced by canine parvovirus. Out of 342 clinically suspected fecal samples collected from the affected parvoviral cases of which 34 fecal samples confirmed of CPV by haemagglutination test (HA) and Polymerase chain reaction (PCR) were propagated in two different cell lines to study the variations in the cytopathic effects caused by CPV. Thirty four samples were propagated in CRFK and only 16 were able to produce minimal cytopathic effect whereas 9 nine samples were infected in MDCK and 5 produced marked cytopathic effect.