Testing seed genetic purity in rice using SSR markers

Abstract

The present study was undertaken with an aim of optimizing SSR marker technology for detecting admixtures and fixing threshold limits of admixture that can be detected using SSR makers. Seeds of two varieties were mixed together in four different ratios and genotyping was carried out using polymorphic SSR markers. The results indicated that the 24:1 ratio i.e. 5% admixtures or offtypes can be detected using this method. The results were further confirmed in other set of varieties and pooling ratios. Results confirmed that this method can be used for detecting offtype admixtures in seed lots using SSR markers. The efficiency of the method can further be increased by increasing the DNA concentration and PCR cycles in the PCR reactions. Thus this method would help in detecting the admixtures in the seed lot and would further help in replacing the present GOT thereby reducing the time, space, labour and especially the environmental influence.by that dilution of phenol, which disinfects the suspension in same time, determines its phenol coefficient.