Phenotypic and genotypic characterization of Campylobacter jejuni from fecal sample of dog suffer from diarrhea

Abstract

Campylobacter has emerged as an important zoonotic food borne pathogen of human and animals worldwide. Campylobacter spp. is frequently isolated from animal, poultry and environmental samples. Campylobacter is one of the most common bacterial enteropathogens of food borne origin in industrialized countries with C. jejuni being the most common species followed by C. coli. The aim of this study was to investigate the incidence of Campylobacter jejuni from dog fecal sample suffer from diarrhea. A total of 19 diarrheal fecal swabs from dog were collected. The samples were screened by cultural examination and studied for biochemical and molecular characterization for confirmation. Total 14 (73.68%) isolates showed typical morphological characteristics on the basis of cultural examination. The isolates were further subjected to phenotypic characterization using biochemical test and genotypic characterization using Polymerase Chain Reaction. The result showed that 8 (42.10%) isolates were found to be positive for C.jejuni. Suggested that close contact with dogs can be recognised as risk factor for human campylobacteriosis from this zoonotic organism.