Bio-control efficacy and chitinase production ability of Trichoderma spp. isolated from saline soil

Abstract

The aim of the study was to assess the potential of Trichoderma spp. isolated from saline soil against predominant soil borne plant pathogens viz., Fusarium oxysporum, Sclerotium rolfsii and Rhizoctonia bataticola.14 Trichoderma isolates were isolated from Amravati, Akola and Buldhana districts located in saline tract of Purna valley in Vidarbha region of Maharashtra state and given code to each isolate. Efficacy of these Trichoderma spp. were tested by employing dual culture technique. All Trichoderma isolates were found significantly effective against tested pathogens. Among the isolates TrJg-11 (Jalgaon jamod) recorded maximum per cent growth inhibition of Fusarium oxysporium (74.79 per cent),TrBt-01 (Bhatkuli) found effective against Sclerotium rolfsii(73.61 per cent growth inhibition) and 66.00 per cent growth inhibition of Rhizoctonia bataticola was recorded by Trichoderma isolateTrDp-04 (Daryapur).The Trichoderma isolates were also assayed for estimation of chitinase enzyme and among the 14 isolates, TrNd-14 (Nandura) found to possessed highest chitinase enzyme i.e. 0.65 units/mg of protein.