Evaluation of interaction potential of Terminalia chebula (Combretaceae) fruit extracts on rat hepatic enzymes

Abstract

The present study was aimed to evaluate in vitro interaction potential of Terminalia chebula fruit powder extracts on rat hepatic CYP enzymes. Alcoholic and hydroalcoholic extracts of Terminalia chebula fruit powder were prepared and standardized for their gallic acid content using RP- HPLC. In vitro metabolism studies were conducted to explore interaction potential of these extracts and marker gallic acid on rat CYP 2D6 enzymes. These extracts and gallic acid were explored for their CYP2D6 enzyme interaction potential by conducting in vitro metabolism study. The study was based on the probe reaction of conversion of dextromethorphan to dextrorphan in presence and absence of extracts. Quinidine sulphate, a known inhibitor, was used as positive control. RP-HPLC with UV detector was used to quantify the metabolite generated in the study. Alcoholic and hydroalcoholic extract fractions and gallic acid in methanol showed inhibitory effect on rat CYP2D6 enzyme activity. Gallic acid proved to be a potent inhibitor of rat CYP 2D6 enzyme.