Molecular identification of Mungbean yellow mosaic India virus (MYMIV) from whitefly and soybean in Jabalpur district of Madhya Pradesh, Central India

Abstract

Yellow mosaic disease caused by genus Begomovirus which is transmitted through whitefly, Bemisia tabaci has become an important constraint for legume production particularly in soybean, urdbean, mungbean and other bean crops of India. The present investigation was performed with the aim to identify Mungbean yellow mosaic India virus (MYMIV) infection in soybean as well as whitefly with the use of coat protein (CP) primer. The genomic DNA templates from both were extracted and amplified with CP primer. DNA templates from yellow mosaic symptomatic plants and carrier whiteflies were amplified with a band size of ~750bp. The present study confirms the utility of CP primers for the detection of MYMIV which is found to be most prevalent yellow mosaic disease in soybean crop at Jabalpur district of Madhya Pradesh, Central India.