To study the isolation of enzyme mannanase for reducing chemical use in paper and pulp industry

Abstract

The present work was carried out to isolate industrially important enzyme producing strain. The soil samples from different areas viz. Rhizospheric, Nonrhizospheric and Landfill areas were collected, appropriate dilution of different soil samples were plated & CFU was calculated. The microbial diversity was also checked & mix culture gram (+)ve & gram (-ve) was observed. These isolates were screen for the presence of various enzymes mannanase, xylanase, laccase, protease & amylase, of the various isolates mannanase producing strains was selected for further study. The sample were plated on a medium with LBG to isolate mannanase producing colony of the RN 11 isolates, strain showing maximum zone of clearance was further selected. Selected strain was subjected to morphological & biochemical characterization which showed the Bacillus species. On the flask level mannanase activity was checked & after 4 hrs enzyme activity 25 IU was observed. The medium was characterized in terms of pH & temperature. Thereafter the strain was used for the bio bleaching of kraft pulp & it reduced the kappa number by 14.86%.