Development and validation of analytical method for simultaneous determination of curcumin and capsaicin in bulk

Abstract

Curcumin and Capsaicin are the natural chemical constituents having potent and synergistic anti-inflammatory activity in combination. In the present analytical research work an attempt has been made to develop and validate simple, selective, specific, precise UV-Spectrophotometric method for simultaneous determination of Curcumin and Capsaicin in bulk. UV-Spectrophotometric method was developed by using methanol as solvent in which Curcumin and Capsaicin showed maximum absorbance at 421 nm and 280 nm respectively. The developed analytical method was validated as per International Conference Harmonization guidelines. Analytical method was found to be linear between the concentration range of 1-5 µg/ml and 25µg-125µg/ml respectively for Curcumin and Capsaicin and also method was found to be selective, specific, precise, robust and rugged with % RSD less than 2%. Hence the newly developed analytical method can be used for simultaneous determination of Curcumin and Capsaicin and its routine quality control analysis in combined bulk.