Evaluation of three different multi-residue pesticide extraction approaches for the analysis of 50 multiclass pesticides from isabgol seeds and psyllum husk

Abstract

Three multi-residue extraction approaches viz. Luke’s, Mill’s and modified Mills approaches were evaluated for their efficiency to extract 50 multiclass pesticides from isabgol seed and psyllum husk. The above approaches were primarily screened by recording their effectively to extract 16 OP pesticides from isabgol seeds. Analytical approaches which are having more pesticides with total error (≤50) were subjected to subsequent cleanup step. The Luke’s extraction approach found unfit for isabgol seeds due to lump formation and poor recovery. Mills and modified Mills approach were tried for their efficacy and found both were suitable for multiclass residue analysis from isabgol seeds. But, later approach was more efficient as 14 out of 16 OPs pesticides fall under accurate extraction category with respect to Mills approach where that was only 2. Modified Mills approach was further tested for GC-ECD amenable pesticide after florisil cleanup in isabgol seed and psyllum husk. This approach proved to be accurate extraction techniques for 34and 44 out of 50 pesticides from isabgol seeds and psyllum husk, respectively.