Isolation, purification and characterization of antibacterial bioactive compounds from Bougainvillea spectabilis Leaf.

Abstract

Bacterial diseases are the major threats for the mankind and silkworm. Hence, present study was initiated to evaluate the antibacterial potential of different solvent extracts of Bougainvillea spectabilis leaves. The initial screening of different solvents against mulberry silkworm infecting bacteria, Bacillus thuringenesis revealed that, chloroform extract showed more inhibition of test pathogen. Further, the chloroform extract was evaluated for the antibacterial potential against Salmonella typhi and Shigella flexneri, human pathogens. Chloroform extract of B. spectabilis inhibited the both pathogens, which confirms the antibacterial character of extract on both gram negative and positive bacteria. TLC fingerprinting of chloroform extract revealed presence of five major bands with different retardation factor value (RF value). The screening of individual bands for antibacterial activity revealed that, the band with Rf value of 0.84. The HPLC profiling of showed the reduction in number of compounds in partially purified extract comparison with crude extract. GC-MS analysis of antibacterial band revealed the presence of Nineteen different compounds having different important biological activity viz., antibacterial, anti-inflammatory, antidiabetic, antifungal, antioxidant and antiprotozoal characters. The results of the present work confirms that, the chloroform extract of B. spectabilis leaf can be used for the management of bacterial diseases of humans and silkworm.