Heamato-biochemical evaluation of midazolam-propofol and xylazine-propofol induction combinations for isoflurane anaesthesia in cattle

Abstract

Twelve animals were randomly divided in to two groups viz., Group-I and Group-II consisting of six animals in each group. Group –I animals received Xylazine (0.08mg/kg) intravenously, 10 minutes later Propofol was given @3mg/kg body weight intravenously for induction of anaesthesia. In Group-II animals’ Medazolam (3mg/kg) was administered intravenously, 10minutes later Propofol was given @3mg/kg body weight intravenously for induction of anaesthesia followed by immediate intubation, maintenance of anaesthesia was done under isoflurane in both the groups. Anaesthetic combinations were compared by haematological and biochemical observations.Haematological observations in the present study revealed that haemoglobin, The haemoglobin (Mean ±SE) in Group-I animals; (10.58±0.63, 10.65±0.55, 09.65±0.45 and 08.90±0.50 respectively) and in Group-II animals (11.25±0.75, 10.78±0.62, 09.65±0.86 and 09.13±0.90 respectively).packed cell volume and total erythrocyte count decreased significantly at maximum depth of anaesthesia in both the groups. Neutrophilia (Mean ±SE values of neutrophils are 32.17±2.04, 34.50±2.06, 42.67±2.35 and 45.67±2.50) with relative lymphocytopenia (Mean ±SE 63.33±2.15, 61.50±2.09, 52.33±2.83 and 49.50±2.72) were recorded in both groups during anaesthesia suggesting, certain amount of anaesthetic stress was produced by these combinations. In both groups most of the biochemical parameters showed the changes, which were within the normal range, suggesting that the anaesthetic combinations used in the present study do not produce adverse effect on these parameters.