Method validation for analysis of pesticide residues in Labeo rohita fish through GC-MS/MS

Abstract

The present study was undertaken to develop and validate multiresidue method (MRM) for twenty four pesticide residues in Labeo rohita fish using Gas Chromatography Triple Quadrupole Mass Spectrometer (GC-MS/MS). The method was based on QuEchERs method (quick, easy, cheap, efficient, rugged and safe) extraction with acetonitrile and dispersive solid phase extraction (d-SPE) cleanup with PSA and MgSO4. At spiking level 0.01, 0.05 and 0.1 µg mL-1 recovery studies were conducted and percent recovery found to be within acceptable limit except synthetic pyrethrnoids at spiking level 0.01 µg mL-1. All RSD values were below 20%. The coefficient of determination (R2) for all pesticide ≥ 0.992. The developed method was validated and found to be fast, simple and reliable for analysis of pesticide residues in fish samples.