Nematicidal effect of certian bio-agents against root-knot nematode, (Meloidoygne incognita) [(kofoid and white) chitwood] on tomato

Abstract

The results of the gram reaction and biochemical tests for the identification of the native isolates of P. fluorescens and B. cereus revealed that all the isolates produced similar results with regard to gram staining (negative), motility (positive), starch hydrolysis (negative), gelatin liquefaction (positive), fluoresent pigmentation (positive) and produced IAA, sidrophore and hydrogen cyanide with little variation. Among the isolates pf5 and Bc6 produced maximum quantity of IAA. All isolates of Paecilomyces lilacinus, colony characters were observed in similar. The morphological characters of M. incognita were observed similar. The culture filtrates of P. fluorescens, B.cereus and P. lilacinus at all conc. caused root-knot nematode mortality and reduced egg hatch count. Maximum inhibition of egg hatch was observed with P. lilacinus at 40 per cent conc. followed by P. fluorescens and. B. cereus. The culture filtrate of P. liacinus isolates was found to posses nematicidal action on second stage juveniles (J2) of M. incognita.