Biochemical characterization and antagonistic potential of Pseudomonas fluorescens against Macrophomina phaseolina

Abstract

The aim of present investigation was to confirm the biochemical characterization and in vitro antagonistic potential of native isolates of Pseusomonas fluorescens against Macrophomina phaseolina. Among the native isolates tested, isolate Pf2 significantly reduced the mycelial growth, size, number and germination of sclerotia of M. Phaseolina. Further, the same isolates tested at different concentrations. Where, isolate Pf2 @ 30% was significantly inhibits the growth (20.45mm) of test pathogen. Also, P. fluorescens showed that all the isolates produced similar results with regard to gram staining (negative), motility (possitive), starch hydrolysis (negative), gelatin liquefaction (positive) and fluorescent pigmentation (positive). Among the isolates Pf2 produced more quantity of IAA (3.6 µg/ml), HCN (8.16) and siderophore (0.89).