Analysis of purified lycopene extract obtained from fresh tomatoes

Abstract

Lycopene is a carotenoid, widely known for its antioxidant properties. In present investigation an attempt was made to observe the effects of purification process on antioxidant activity and isomerisation of lycopene. Antioxidant activity of crude as well as purified lycopene extract was measured as Trolox Equivalent Antioxidant Capacity (TEAC) using ABTS (2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate) method. Crude as well as purified lycopene extract were subjected to spectrophotometric analysis for identification of peaks. HPLC analysis of crude as well as purified lycopene extract was carried out for cis – trans identification of peaks. Purification process significantly (p