Anti-proliferative activity of L-Asparaginase enzyme from fungi on breast cancer

Abstract

The present investigation deals with the anti-proliferative activity of an extracellular enzyme known as L-Asparaginase from Aspergillus fumigatus screened from infected leaves of the medicinal plant Annona muricata. The anti-proliferative activity of L-Asparaginase from Aspergillus fumigatus was checked on human breast cancer cell line MDA-MB-231.The influence of pH, temperature, substrate concentration, incubation period and concentration of metal ions were analyzed in the production of L-Asparaginase. The highest production of enzyme noticed at a pH of 8, 20mM substrate concentration, 20 minutes of incubation period, Presence of Fe2+ ions and 40 0C of temperature. These were the optimum conditions for the effective yield of enzyme. The enzyme isolated from Aspergillus fumigatus is a promising anti-proliferative agent and can be used in the expansion of further compositions for the therapy of tumors.