Studies on the efficacy of different methods of nematode extraction with special reference to soil types

Abstract

A study was conducted to know the efficiency of different nematode extraction methods from soil. Among different Pre-soaking times for extraction of nematodes from the soil by modified Baermann funnel method, Pre-soaking for 30 minutes showed increased recovery of nematodes from black soil (20.02% increase) and red loam soil (25.21% increase) respectively, when compared with the regular 5 minutes Pre-soaking with recovery of different nematode genera viz., Longidorus elongatus (58.33 percent) and Pratylenchus coffeae (41.00 percent) in both black and red loam soil respectively. Thirty minutes Pre-soaking was found to be the best for extraction of nematodes by centrifugal sugar floatation method from both black and red loam soil with 22.16 percent increase in nematode recovery. Among the extraction of nematodes by incubation technique with different mistifying intervals, mistifying with 5 minutes interval was found to recover the highest number of nematodes, which recorded 40.56 and 48.32 percent increased nematode recovery in both black and red loam soil respectively, when compared to 30, 60, and 120 minutes of spraying interval.