Radio-sensitivity of nodal segments of grape cv. ‘Red Globe’ to gamma rays under in vitro

Abstract

In order to ascertain the Irradiation sensitivity of grape cv. Red Globe, nodal segments were subjected to Gamma irradiation treatments (0, 3, 6, 9, 12, 15, 18, and 21 Gy) and inoculated on MS medium supplemented with BAP 2mgL-1. The cultures were sub-cultured on the MS modified medium up to three cycles (M1V3) and rooting was induced in altered MS medium supplemented with IBA 2mgL-1 and activated charcoal 200 mgL-1. The in vitro rooted plantlets were transferred to pots in polyhouse for hardening. Morphological observations revealed the mean explant survival was highest in 3 Gy dose (96.5%) among the irradiation treatments. However, as the irradiation dose was increased further, there was significant decrease in explant survival, regeneration rate, days to bud break and multiple shoot induction, number of shoots per explants, days to root initiation, shoot and root length along with significantly enhanced in vitro shoot and root abnormalities. The gamma irradiation dose of 18 Gy which resulted in 52.00% survival along with significant in vitro shoot abnormalities (33.75%) was adjudged as the lethal dose 50 (LD50).