In vitro bio-efficacy of bioagents and botanicals against Macrophomina blight of mungbean caused by Macrophomina phaseolina (Tassi) Goid

Abstract

All the seven fungal and two bacterial bioagents / antagonists evaluated in vitro were found antifungal / antagonistic against M. phaseolina. However, T. harzianum was found most effective and recorded significantly highest mycelial growth inhibition (77.59%) of the test pathogen over untreated control. The second and third best bioagents / antagonists found were A. niger and T. viride which recorded mycelial inhibition of 68.17 and 65.46 percent, respectively. The antagonists P. fluorescens and B. subtilis was also found fungistatic and recorded 51.37 and 60.90 percent mycelial inhibition, respectively. Also all the 12 botanicals / plant extracts evaluated were found fungistatic / antifungal against M. phaseolina. Average mycelial growth inhibition recorded with all the test botanicals was ranged from 6.12 (L. carnea) to 95.08 (A. sativum) percent. However, significantly highest average mycelial growth inhibition was recorded with the botanicals A. sativum (95.08%) and A. cepa (93.08%). This was followed by the botanicals viz., A. indica (67.43%), D. metal (59.24%), and I. carnia (6.12%) was found less effective.