Evaluation of antiangiogenic and antigenotoxic potential of green and black tea extracts by chicken chorioallantoic membrane assay

Abstract

Present work deals with analysis of the antiangiogenic and antigenotoxic properties of green tea and black tea extracts by chicken chorioallantoic membrane (CAM) assay in vivo and by radical scavenging assay. The extracts of green tea and black tea prepared in water were tested, administering at 48, 72 and 96 hrs. of incubation to observe angiogenesis of the CAM at 144 hrs. of development and antigenotoxic properties using radical scavenging assay. The extracts of green tea and black tea reduced neovascularization. Green tea extract showed highest inhibitory activity in angiogenic responses as compared to black tea. The quantitative analysis indicated inhibition in elongation and proliferation of both secondary and tertiary vessels. It seems to be the consequence of interference of extracts in signaling of angiogenic agents from epithelial cells or cellular apoptosis, which in its absence results in normal CAM angiogenesis. The result claims strong antiangiogenic and antigenotoxic properties of the green and black tea.