Studies on superoxide anion radical scavenging activity, antioxidant activity, and reducing power of methanolic and aqueous extract of different medicinal plants

Abstract

Present investigation entitled Studies on superoxide anion radical scavenging activity, antioxidant activity, and reducing power of methanolic and aqueous extract of different medicinal plants was carried out during December 2016 - April 2017 in Department of Biochemistry and Molecular Biology, MGM College of Agricultural Biotechnology, Gandheli, Aurangabad. Experiment was laid out in Factorial Randomized Block Design (FRBD) with ten treatments of different medicinal plants (Amla, amsul, chinch-Imli, neem, gavti chahaa, pipal, umber, wad, Pudina, coriender) and three replications. The healthy disease free medicinal plants were selected. The antioxidant activity of the medicinal plants extract and the standard were assessed on the basis of the radical scavenging effect of the stable 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) free radical activity by modified method. The dilute working solutions of the test extract were prepared in methanol. Ascorbic acid was used as standard in 1-100µg/ml solution. 0.002% of DPPH was prepared in methanol. These solution mixtures kept in dark for 30 min and optical density was measured at 517nm using Cecil-Elect Spectrophotometer. Methanol (1ml) with DPPH solution (0.002%, 1ml) was used as a blank.