Role of brassinolide on physio-biochemical traits of barley under salt stress

Abstract

The study entitled “Role of Brassinolide on Physio-biochemical Traits of barley (Hordeum vulgare L.) Under Salinity’’ was conducted in the cage house at Department of Plant Physiology, S.K.N. College of Agriculture Jobner during rabi season of 2015-2016 under pot culture experiments. Two barley cultivars namely RD 2035 (salinity susceptible) and RD 2794 (Salinity tolerant) were grown in cemented pots under salinity (0, 3, 6, 9 and 12 dSm-1). Different concentrations of brassinolide (0.0, 0.5, 1.0 and 1.5 ppm) were sprayed at 45 and 75 days after sowing. Control plants were provided normal water. Physio-biochemical observations were recorded at 52 and 82 days after sowing in pot conditions. A significant decrease were recorded in Chlorophyll, protein, relative water content, cell membrane stability, with increase in salt stress up to EC 12 dSm-1.Whereas the foliar spray treatment with brassinolide up to 1.5 ppm significantly increased chlorophyll, proline, protein, reducing sugar, relative water content, cell membrane stability in both the cultivars at 52 and 82 DAS under salt stress as well as non stress conditions. The 1.5 ppm concentration of brassinolide was found most effective under salt stress and non stress conditions. RD 2794 observed superior over RD 2035 on the basis of physio-biochemical analysis.