Expression studies of fowl adenovirus penton base

Abstract

The fowl adenovirus infections are responsible for high morbidity and high mortality in poultry flock throughout the world. There is no effective vaccine against these viruses. The present study was conducted to express penton base gene which is one of the major immunogenic proteins of virion. The penton base gene was amplified and cloned into plenti6/V5-D-TOPO vector system which is HIV-1 based vector. After transfection, the pseudotyped lentivirus particles were synsthesized which were found to be able to transform as into vero cells. The expression of penton base protein in vero cells was observed by immunofluorescence studies.