SNP discovery and association of S100A8 allelic variants with incidence of clinical mastitis in Sahiwal and Karan Fries cattle

Abstract

The present study was carried out to identify single nucleotide polymorphisms (SNPs) in bovine S100A8 gene and associate novel allelic variants with mastitis incidence in Sahiwal and Karan Fries cattle. A panel of unrelated cattle was subjected to targeted sequencing to detect a total of 23 novel SNPs within S100A8 gene. A novel PCR-RFLP assay (HpyCH4V) was developed to genotype -2 (A/G) SNP locus in 148 Karan Fries cattle and 147 Sahiwal cattle. The genotype and allele frequencies were calculated for each of the four groups of animals viz. affected Sahiwal cattle, unaffected Sahiwal cattle, affected Karan Fries cattle, unaffected Karan Fries cattle. The overall frequency of GG, GA and AA genotypes were 0.884, 0.102 and 0.013 respectively in Sahiwal cattle while it was 0.973, 0.02 and 0.007 respectively in Karan Fries cattle. No significant association of S100A8 genotypes with incidence of clinical mastitis (P>0.05) was observed.in investigated cattle breeds.