Effect of tris egg yolk citrate (TEYC) supplemented with tomato juice on spermatozoa extracted from epididymis and preserved at refrigerated temperature of non-descriptive breed of bucks

Abstract

The objectives of the study were to determine the effects of tomato juice as additive on sperms characteristics retrieved from epididymis of non-descriptive breed of slaughtered bucks during in vitro storage at refrigerator temperature. Eight pair of testes from slaughtered buck was collected and spermatozoa were retrieved from the epididymis at 37 oC after adding TEYC dilutor and spermatozoa were fixed at the concentrations of 300x106/ml. They were divided into five groups; control (T1) and treatments T2, T3, T4 and T5 using diluted spermatozoa with 4, 6, 8 and 10 ml of tomato juice per 100 ml of diluent, respectively. Individual motility, live spermatozoa, normal spermatozoa, HOST reacted spermatozoa and motility degeneration rate were observed in vitro storage of refrigerated temperature at 0, 12, 24, 36 and 48 h. No significant (p>0.05) improvement was observed by adding diluent into collection tubes either supplemented before and after refrigerated temperature. The study also revealed a highly significant (p< 0.01) correlation between the various spermatozoa evaluation parameters. In conclusion, addition of diluent with different treatment groups (4%, 6%, 8% and 10% added tomato juice) the Spermatozoal sample if used within 12 h in 4%, 6% and 8% tomato juice added treatment groups, the motility remained up to 50% that could be utilized for fertility purpose and the correlation coefficient of all the parameters indicated the retrieved spermatozoa was potential for fertility.