Screening and molecular identification of microbial strain Bacillus axarquiensis P6 from algal biomass for production and optimization of multiple enzymes of biotechnological interest

Abstract

This study is a first of its own kind for isolation of multiple enzyme producers from biomass of Rhizoclonium sp. algae collected from different sites of Himachal Pradesh. One of the isolated 19 strains, the isolate P6 exhibited zones of hydrolysis for cellulase, xylanase and amylase activity indicating its potential for multiple enzyme production. Isolate identified based on their morphological and physicochemical characteristics and 16s rRNA sequencing. Phylogenetic analysis based on the results of 16S rRNA gene sequencing revealed that P6 was in close identity to Bacillus axarquiensis. Then different parameters for enhanced enzyme production were optimized using classical one factor at a time approach (OFAT). The effects of different media, medium pH, temperature, inoculum size and incubation period for multiple enzyme production were studied. The enzyme production was found maximum at pH 8.0 for cellulase and amylase while pH 5.0 for xylanase, temperature 30 oC and incubation time 72 h by multiple enzyme producer Bacillus axarquiensis P6. Overall, the results of the present study demonstrate that the genus Bacillus axarquiensis P6 is a potential candidate for multiple enzyme production and can be used for various biotechnological purposes as hydrolysis of biomass for biofuel production.