DNA fingerprinting of soybean (Glycine max l.) Genotypes by using simple sequence repeats (SSR) markers

Abstract

Thirteen soybean [Glycine max (L.) Merr.] genotypes were screened using 52 SSR markers. Out of that, 31 markers were recognized to be polymorphic across the thirteen soybean genotypes under study. A total of 103 polymorphic alleles with an average of 7.92 alleles per locus were detected with maximum eleven bands (primer Sat_406) and minimum one polymorphic allele (Satt126). The pair wise coefficient of genetic similarity between all soybean genotypes ranged from 0.792 to 0.929. Out of 31 polymorphic SSR primers, fifteen SSR primers (i.e. Satt184, Satt329, Satt335, BE806308, Satt077, Satt557, Satt194, Sat_409, Satt328, Satt406, Satt717, Satt329, Satt120, AW620 and Sct_189) successfully distinguished and discriminated soybean genotypes by generating unique allele for respective genotype. These unique bands can be used for the identification of specific soybean genotype.