Different histochemical observations on the kidney of guinea fowl (Numida meleagris)

Abstract

The Guinea fowl kidney was histologically observed after different components including capsule, cortex, medulla and different parts of uriniferous tubules. The mucopolysaccharide enzymatic activity in the kidney was revealed by PAS-AB (Periodic Acid-Schiff- Alcian blue) method. The PAS positive activity was witnessed at mesangial cells of renal corpuscle and basement membrane of the distal convoluted tubule while the Alcian blue reaction was positive in most of the components while the cortical and medullary collecting tubule showed intensively positive reaction. The acid phosphatase, alkaline phosphatase and succinic dehydrogenase methods were also performed. The acid phosphatase has shown its intensified activity in parietal & visceral layer of renal corpuscle and lumen & basement membrane of proximal convoluted tubule whereas, the alkaline phosphatase was showing the moderate activity in all components of the kidney. The Intense activity of succinic dehydrogenase was observed in the proximal & distal convoluted tubule and the collecting tubule except the renal corpuscle which showed a weak activity.