Effect of organic trace mineral supplementation on expression of CxCLi2 gene in broilers

Abstract

It is well recognized that demand of poultry meat and meat products has increased due to shortage of red meat supply and price phenomena. Annual broiler global meat production was 84.6 million tons in 2013 and covered 33% of global meat demand. It is well established that trace minerals are important for broiler normal growth and development. However, due to relative less economic importance, trace minerals sector failed to attract poultry scientist attentions. The current interest in trace minerals nutrition has been vigorously undertaken due to better bioavailability of trace minerals and concerns of environmental pollution. Hence, in the present investigation, the effect of organic trace minerals supplementation on expression of CxCLi2 gene in broilers was attempted. A total of 162 broiler birds randomly divided into nine groups were used for the experiment. Broilers were divided into 09 groups and each group consisting of 18 birds in 3 replicates. T1 group was kept as control. T2, T3 group was supplemented with zinc (40 mg/kg of feed) from inorganic and organic form respectively. T4, T5 group was supplemented with selenium (0.3 mg/kg of feed) from inorganic and organic form respectively. T6, T7 group was supplemented with chromium (2 mg/kg of feed) from inorganic and organic form respectively. T8, T9 group was supplemented with combination of all 3 minerals from inorganic and organic form respectively. RT-PCR expression analysis of CxCLi2 gene in spleen revealed that maximum up regulation (8.06 fold) was found in T5 group, followed by T9 (7.25 fold) whereas in bursa of fabricius the maximum up regulation (13.57 fold) was found in T9 group followed by T5 (10.10) fold as compared to control group.