Variability in Sclerotinia sclerotiorum of mustard in northern region of M.P. (India)

Abstract

Stem rot incited by Sclerotinia sclerotiorum (Lib.) de Bary is one of the drastic disease of mustard and it causes quality and quantity losses in mustard. The five isolates of S. sclerotiorum from different geographical locations of northern region of Madhya Pradesh were collected and compared for their cultural and morphological characters under artificial culture conditions based on radial growth. Three degenerated primers of arbitrary reverse transcription sequences produced 340 scorable amplicons through florescent labelled random amplified polymorphic DNA markers. Nucleotides range were designed for sizing DNA fragments in the 35-500 nucleotides range and provides 16 single-stranded labeled fragment. Out of these, 251 fractionated fragments were reported polymorphic. Hence, the present investigation confirmed the diversity among the different geographical isolates from five districts of northern region of Madhya Pradesh (India).