In vitro evaluation of antagonistic potential of native Trichoderma spp. Against Lasiodiplodia theobromae causing Tree bean decline in Manipur

Abstract

The antagonistic potentialities of nine species of native Trichoderma viz., T. asperellum (KU933475), T. koningiopsis (KU904460), Hypocrea lixii (KX0113223), T. harzianum (KU904458), T. ovalisporum (KU904456), T. atroviradae (KU933472), were evaluated in vitro against Lasiodiplodia theobromae causing tree bean decline in Manipur. The current study of dual culture assay, revealed the percentages of mycelial growth inhibition of L. theobromaeby T. asperellum (KU933475), T. koningiopsis (KU904460), Hypocrea lixii (KX0113223), T. harzianum (KU904458), T. ovalisporum (KU904456), T. atroviradae (KU933472),T69 T. harzianum (KU933468), T. asperellum (KU933476), T. harzianum (KU933471) were 55.29%, 61.57%, 56.86%, 72.94%, 62.75%, 60%, 57.25%, 51.76% and 61.18% respectively. All the species considerably inhibited the growth of Lasiodiplodia theobromae pathogen. The outcomes direct that the extent of inhibition by all the nine species of Trichoderma provides use of excellent potential antagonists capable of reducing the growth of Lasiodiplodia theobromae on tree bean decline.