GC-MS analysis of the hexane extract of Limnophila indica (L.) Druce, its total phenolics, in-vitro antioxidant, anti-inflammatory and antifeeding activity against Spilosoma obliqua

Abstract

The present study was carried out for analysis of hexane extract of Limnophila indica and evaluated for total phenolics, in-vitro antioxidant, anti-inflammatory and antifeeding activity. The n-hexane extract was obtained by Soxhlet apparatus and analysed by GC-MS. GC-MS analysis leads to identification of thirty-eight compounds in extract comprising of 83.9% of total extract composition, containing aristolone (40.3%) as the major compound. Phytochemical analysis resulted in potentially significant total phenolics, total flavonoids and total ortho-dihydric phenol content. Antioxidant activity of extract assessment using NO, DPPH radical scavenging, metal chelating and reducing power activity resulted significant antioxidant activity presumably due to qualitative and quantitative difference of their antioxidative components. Also concentration dependent inhibition of protein (albumin) denaturation exhibited in assessment of in-vitro anti-inflammatory activity in the extract. Percent antifeeding analysis on Spilosoma obliqua results into higher range of inhibition of feeding action in a dose dependent manner and more prominent at higher concentrations (20 and 25 ppm).