Assessment of genetic diversity in garlic (Allium sativum L.) genotypes based on ISSR markers

Abstract

Genetic diversity among one hundred and thirty-one accessions of garlic was analyzed using Inter-simple sequence repeat (ISSR) markers. A total of twelve polymorphic bands were detected with four primers. Genetic similarity result showed genetic variation among the garlic accessions ranged from (0.00) to (1.00) with a mean of 0.501. All 131 accessions were clustered into two major groups. The smallest genetic similarity value was observed between PG-20 and all genotypes except K1 (0.500) and W6-12840 (0.125) genotypes, which appear as the most dissimilar accessions and distantly related. The maximum genetic similarity value of 1.00 was obtained between PG-32 with F4, F5, BG-108, F2, CGF-2 genotypes. The genotypes PG-20, K1, WG-35698, Single Kale, GG-4, RG-77, CFG-3, G-335, and AC-200 are identified as more diverse among the genotypes under study could be used for the genetic improvement of garlic cultivars.