Optimization of process parameters through plackett-burman design by Paenibacillus polymyxa G7 isolated from resins of Pinus roxburghii

Abstract

Lipases are ubiquitous in nature and high demanded industrial catalysts. In the present study, the cultural conditions of extracellular lipase production from a bacterial strain Paenibacillus polymyxa G7 were statistically optimized by using Plackett-Burman design under submerged fermentation conditions. Maximum enzyme activity was noted in a medium containing; galactose (0.2%), ammonium sulphate (0.5%), Tragacanth gum (1.5%), yeast extract (0.25%), incubation time (4 days), temperature (40 oC), pH 10.0 with an inoculum size 10%. As witnessed from Pareto chart; variables that were most important for lipase production were temperature and incubation time (days) whereas other parameters showed minute effect for lipase production.