Ultrasound assisted extraction of bio-colorant from walnut hull

Abstract

Walnut hull is considered as agricultural waste which is considered as the rich sources of bio colorant and bio active compounds. Bio colorant can be used as food additive to replace synthectic dyes and colorants. At present, colorant extraction is done by utilizing hot water for a long duration of time which is a time-consuming process. However, it is verified that Ultrasonication lessens the extraction time and maximize yield as compared to the traditional method in use. Identifying the capability of Ultrasound assistance in extraction, this research emphasizes on bio colorant extraction using walnut hull as raw material. Walnut hull converted into the powdered form was utilized for extraction of biocolorant. It was extracted with different combinations of Ultrasound Power (150, 200,250 W), treatment time (10, 20, 30 mins), solvent volume (20, 30, 40 ml/g) and particle size (150, 300, 450 µm).As a result, the highest yield of 49.76% was attained at the Ultrasound power of 200 W Ultrasound Time of 20 Minutes, solvent volume 1:20 and particle size 150µm. The research confirms that utilizing Ultrasound enhanced yield and also saves sufficient amount of time and energy.