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Serological and molecular detection of Potato Leaf Roll Virus (PLRV)
Journal of Entomology and Zoology Studies · 2017 · Vol. 5(3) · pp. 1367–1373
Hidayat Ullah Khan✉Ikram UllahQazi Sami ul HaqZiafat RehmanShafiq Ur RehmanMuhammad Ayub JaddonAhsan UllahAzam HayatSidra KhanMujaddad Ur RehmanInam UllahNighat Sultana
Abstract
The purpose of the present study was to confirm the presence of PLRV by DAS-ELISA and RT-PCR. For this the present study undergo serological and molecular detection of potato leaf roll virus (PLRV) by studying PLRV on the basis of symptomlogy, by serological detection (DAS-ELISA) and amplification of coat protein gene by RT-PCR. Ten (10) samples of potato were tested and 8 were found to be infected either by a single virus or more than one virus. Eighty (80)% infection was observed by DAS-ELISA and the resulted cDNA was used in PCR reaction using gene specific primer pair an amplified product of approximately 626 bp was observed.
Plant Virus Research StudiesPlant Disease Resistance and GeneticsPlant Disease Management TechniquesPotato leafroll virusSerologyBiologyVirologyPrimer (cosmetics)VirusComplementary DNAGenePolymerase chain reactionPlant virus
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