A comparison of stress related biochemical changes in broilers reared under coloured light emitting diodes Vis-À-Vis incandescent supplemental lighting

Abstract

Present study was conducted to compare the biochemical parameters of broiler birds’ in an open-sided house under supplemental lighting programme using light emitting diodes (LEDs) and incandescent light. For this purpose 240 straight run commercial ven cobb broiler chicks were procured and distributed in four different light treatment groups i.e. T1; white (650nm), T2; green (565nm), T3; blue (430nm) light LED (3 Watt each) and incandescent light bulbs (60 Watt each) as control (Tc) in 12 pens (4x3 factorial design). At the day time, open-sided house was open from 10.00 AM to 04.00 PM and rest of the time sides of the house was covered with the black coloured tarpaulin sheet. Biochemical parameters (serum Ig, glucose, total protein, BUN, GPX, SOD, catalase and lipid peroxidation) were estimated from randomly collected blood from 3 birds of each replicate of each treatment at 7th, 21st and 42nd day of experiment period. Blood biochemical evaluation indicated that the level of total immunoglobulin at 6th week was significantly (p≤ 0.05) higher in T3 (2.27±0.12) and T2 (2.52±0.09) LED light treatment, whereas, catalase (7.25±0.11) was significantly (p≤ 0.05) higher in control group as compared to LED treatment groups. Interleukin 1β were estimated using bioassay technology laboratory enzyme- linked immunosorbent assay (ELISA) kit and the value on 21st day of experiment period was highest in T1 group (3104.68±149.27) and T3 group (1970.42±227.02) differ significantly (P