Molecular characterization of Fusarium oxysporum f. sp. melongenae (Schlecht) mutuo and Ishigami in Marathwada region of Maharashtra by using ITS-RFLP Markers

Abstract

ITS-RFLP (Internal Transcribed Spacer-Restriction Fragment Length Polymorphism) marker were used to study the genetic variability among Fusarium oxysporum f. sp. Melongenae isolates from different regions of Marathwada collected during the summer 2018-2019. A total 2 restriction enzymes were used for the digestion of ITS region of the Fom isolates Study of ITS-RFLP analysis for genetic diversity shows the genetic relationship between the 8 Fom isolates. The major cluster I consisted of seven isolates viz. Fom-1 (Soegaon), Fom-2 (Ambajogai), Fom-4 (Badnapur), Fom-5 (Chakur), Fom-6 (Kinwat), Fom-7 (Tuljapur) and Fom-8 (Gangakhed) while second major cluster II comprises single isolates Fom-3 (Sengaon) shown 33% genetic similarity with cluster I and genetic diversity 67% with all isolates.