Genetic diversity of whitefly, Bemisia tabaci population of Rajendranagar, Telangana, India

Abstract

The genetic identification of whiteflies collected from the different fields of Rajendranagar, Hyderabad, Telangana, India during summer 2017 was done by SSR-PCR of the mitochondrial cytochrome oxidase I (mtCOI) gene. The results obtained from the present study revealed the amplification of mitochondrial cytochrome oxidase I (mtCOI) gene fragment using the primers (C1-J-2195 and L2-N-3014) produced Bemisia tabaci specific ~860bp band in all the samples of B. tabaci. MtCOI gene of this B. tabaci samples collected from Rajendranagar, Telangana were compared with other known sequences in GenBank and the test samples showed high level of nucleotide and amino acid identity with Asia I and Asia II-1 genetic groups. The phylogenetic tree constructed based on UPGMA (Unweighted Pair Group Method with Arithmetic Mean) analysis with 10 mtCO1 sequences of B. tabaci samples also revealed that Rajendranagar B. tabaci population clustered with sequences corresponding to Asia-I and Asia II-1 genetic groups. So far there is no report on the presence of Asia II-1 genetic group from the Telangana state.