Evaluating the efficacy of different bio control agents against Magnaporthe grisea under in vitro condition

Abstract

Rice blast causes yield losses to Basmati rice farmers in worldwide. Although this problem is currently being addressed through the use of resistant rice varieties, fungicide and crop rotation farming, these methods alone do not form a durable, long lasting solution in mitigating disease. Here Antagonistics activity of different bio-agent such as Chaetomium globosum, Trichoderma viride, Bacillus subtilis, Trichoderma harzianium and Pseudomonas fluorescens tested their efficacy as bio-control agents against to Magnaporthe grisea. Trichoderma viride, Trichoderma harzianium and Chaetomium globosum is known for its mycoparasitic properties, whereas Bacillus subtilis and Pseudomonas fluorescens have antagonistic mechanism for the control of fungal disease. The results showed that the dual inoculation of bio-control agents caused significant (p ≤0.05) inhibition of M. grisea as compared to a single agent. The100% inhibition of the fungal (M. grisea) radial growth was recorded C. globosum at different time intervals 72, 96 and 120hrs.