Assessment of antioxidant effect of biochanin: A on isoprenaline-induced cardiac fibrosis in mice

Abstract

Biochanin-A, an is flavonoid abundantly existed in red clover and peanuts, has been reported to possess anti-inflammatory and anti-proliferative effects in different conditions. However, protective antioxidant effects on isoprenaline (ISP)-induced cardiac fibrosis in mice model have not been reported. Thus, the present study was undertaken to assess the antioxidant effect of biochanin-A against ISP-induced cardiac fibrosis in mice. Cardiac fibrosis was induced by administration of ISP @ 20 mg/kg body weight via subcutaneous (S/C) route for 14 days. Biochanin-A @ 30 mg/kg (orally) was co-administered with ISP (S/C) in treatment group for 14 days. Serum samples from different groups were assessed for catalase, superoxide dismutase (SOD), reduced glutathione and MDA activity. Serum level of catalase, SOD, reduced glutathione and MDA levels were altered following ISP-induced cardiac fibrosis which have been improved by biochanin-A-co-administration. In conclusion, biochanin-A may have potential to check progression of ISP-induced myocardial fibrosis due to its anti-oxidative effects.