Effect of interspecific protoplast fusants strain (Trichoderma viride and Trichoderma koningii) on Arachis hypogaea L. for zinc tolerance using physiological and biochemical traits

Abstract

This study was designed to investigate the efficacy of three fusant strain of Trichoderma koningii and Trichoderma viride on pot culture experiment using Arachis hypogeal L. for metal tolerance using physiochemical and biochemical traits against parent Trichoderma strain. The zinc toxicity at all concentration (100, 500, 1000, 1500 and 2000 ppm) exhibited significant reduction in chlorophyll, membrane stability index and biomass in groundnut seedling. While fusant strain treated plants under zinc stress showed that increase the chlorophyll, membrane stability index and biomass in groundnut seedling. The enzyme activity in fusant treated plants was always lower compared to parent and control plant. The groundnut seeding treated with fusant strain showed higher biomass, on the other hand non treated plant and parent strain treated plant gained higher biomass. These results indicate that fusant strains possess greater tolerance capacity than parent strain. And this technique feasible for improved biocontrol strain.