Optimization of protocols for conversion of porcine urinary bladder into bladder acellular matrix graft (BAMG) using biological detergents and enzymes

Abstract

The present study was carried out for optimization of protocols for conversion of porcine urinary bladder into bladder acellular matrix graft (BAMG) using different biological detergents and enzymes for tissue engineering. The tissue treated with enzyme and biological detergents at different concentration showed more dissolution and only few remnants of tissue were left and were not appropriate for grafting purpose. Therefore, enzyme treatment was omitted and tissue was directly treated with different concentrations of biological detergents for different time intervals. In the present study, the bladder graft treated with 0.5% SDS for 24 h showed complete acellularity with normal collagen fibres arrangement.