Detection of Brucella spp. in spiked clinical sample and bovine aborted foetal stomach contents by polymerase chain reaction

Abstract

Present study was aimed to develop a PCR targeting insertion sequence IS711 to detect Brucella organism. This insertion sequence is considered as conserved and evolutionary stable nucleotide sequence among species in Brucella genus. This PCR assay could detect 150fg /µl B. abortus S99 genomic DNA, equivalent to 100 Brucella organism. This PCR assay did not show any cross reactivity with other related gram negative bacteria or micro-organism responsible for abortion in cattle. This PCR assay could detect Brucella organisms in spiked clinical sample (108 cfu/ml) and bovine aborted foetal stomach contents. This PCR may be used as an alternative of isolation method to detect Brucella organism in clinical sample.