Callogenesis and multiplication of Lepidium sativum from shoot tip explants under in vitro condition

Abstract

An experiment was carried out to establish an efficient callus induction system and multiplication of Lepidium sativum. Among the different explants derived from leaves, root, hypocotyl and shoot tip. The best callus initiation performance was observed by shoot tip (96%). 15 media combinations of Murashige and Skoog basal media supplemented with varying concentration of cytokinin and auxin were evaluated. All media combination produced calli in varying frequencies. However, culture media combination MS5B1N (90.00%), MS5B1N (81.00%) MS3N (84.50%) was found to be most responsive for callus induction, closely followed by MS5D (78.00%). This procedure can be advantageously of extraction of active ingredient as well as developing of in vitro regeneration protocol that can use in genetic improvement of this multipurpose medicinal plant.